Biothon - Synthetic Biology

A comprehensive study collection for the Biothon biology competition covering synthetic biology. Topics span foundations of synthetic biology (central dogma as engineering framework, standardized biological parts/BioBricks, chassis organisms), genetic circuit design (core genetic parts, logic gates, toggle switches, advanced circuit architectures), metabolic engineering (pathway design, biofuel production, natural product synthesis and genome mining), cell-free systems and minimal cells (TX-TL systems, minimal genomes, xenobiology and genetic code expansion), directed evolution and protein engineering (random mutagenesis, rational/computational design, display technologies), biosensors and diagnostics (whole-cell biosensors, cell-free paper-based diagnostics, CRISPR detection, synthetic gene networks), and applications/ethics/biosafety (biomanufacturing, environmental remediation, biosafety/biosecurity). Designed for advanced high school students with emphasis on engineering principles, cutting-edge technologies, and real-world applications.